PROTECTIVE ROLE OF VITAMIN E AGAINST THE HARMFUL IMPACT OF CHLOROPYRFIOS ON SEMEN QUALITY AND TESTOSTERONE LEVELS OF RABBITS.

Abstract

Chlorpyrifos (CPF) is a broad spectrum organophosphate pesticide used for agricultural health purposes. Vitamin E has been reported to be important antioxidant. Therefore, this study aimed at elucidating the protective effects of V.E  against reproductive toxicity of CPF  in male rabbits. Therefore, the present experiment was undertaken to determine the effectiveness of vitamin E in alleviating the toxicity of chloroyfiros   on  body weight, reproductive performance and  testesteron  of male rabbits. Animals were assigned to 1 of 4 groups: control; 33.3 mg CPF mg/kg bw; 100 mg V.E/kg bw; CPF (33.3 mg/kg bw) plus V.E  (100 mg/kg bw), respectively. Results showed that live body weight (LBW),  testes weight (RTW), and serum testosterone were significantly reduced (P<0.05) by treatment with CPF  treatment also decreased (P<0.05) ejaculate volume, sperm concentration, total sperm output, sperm motility index, and semen initial fructose concentration. The negative effects of CPF on semen characteristics were dose-dependent. Treatment with V.E increased (P<0.05) LBW, RTW andserum testosterone concentration, improved semen characteristics, and alleviated the negative effects of CPF treatment increased (P<0.05) the numbers of abnormal and dead sperms in a dose-dependent manner. Treatment with V.E alleviated the negative effects of CPF during treatment . Results demonstrated the beneficial influences of V.E  in reducing the negative effects of CPF on production and reproduction of male rabbits.