Comprehensive Review of Transcriptome Analysis Techniques

Abstract

Transcriptome analysis has become a fundamental tool in human genetics research, enabling the comprehensive exploration of gene expression patterns and their potential implications in various biological processes and disease states. This review provides a comprehensive overview of the latest advances in the primary transcriptome analysis techniques, including RNA sequencing (RNA-seq), microarrays, and other high-throughput gene expression profiling methods. The strengths, limitations, and applications of each approach are thoroughly examined, highlighting their unique capabilities and suitability for specific research objectives within the field of human genetics. The review delves into the underlying principles, methodological advancements, and the diverse range of applications that have emerged, empowering researchers to make informed decisions in selecting the most appropriate transcriptome analysis technique for their investigative needs.

Keywords: Transcriptome analysis, RNA sequencing (RNA-seq), microarrays, gene expression profiling, human genetics, high-throughput technologies, bioinformatics, transcriptomics.