Study the antimicrobial activities of Russian olive (Elaeagnus angustifolia L. fruit)-based mouthwash on bacteria isolated from dental caries; A preliminary survey

Abstract

Background: Elaeagnus angustifolia also known as Russian olive is an important medicinal fruit with high antimicrobial and antioxidant effects. In the present survey, the antimicrobial effect of E. angustifolia fruit-based mouthwash was tested against bacteria isolated from dental caries. The cotton swab was used to sample the dental caries areas in the oral cavity. Methods: Samples were cultured and isolated bacteria were subjected to antimicrobial assessment. E. angustifolia mouthwash was produced with a routine laboratory formula using the E. angustifolia ethanolic extract. Then, its antimicrobial effects were assessed using disk diffusion and Minimum Inhibitory Concentration. Results: S. mutans (25%), E. cloacea (15%), and S. aureus (20%) were isolated from dental caries samples. E. angustifolia mouthwash (1%) harbored the highest antimicrobial effects against S. mujtans (15.61±0.94 mm), and S. aureus (12.01±1.10 mm), while showed the lowest against E. cloacea (9.73±0.27 mm). Findings showed that the lowest MIC levels were obtained for S. mutans (2 mg/ml). The highest MIC level was found for E. cloacea (8 mg/ml). Conclusion: E. angustifolia mouthwash can be effectively used as a novel mouthwash with high antimicrobial effects against the pathogens responsible for dental caries. However, additional clinical trials should be done to distinguish other aspects of the E. angustifolia mouthwash in the oral cavity.

Keywords: E. angustifolia mouthwash, Dental caries, Antimicrobial effects.